Journal of Nepalese Prosthodontic Society



Introduction: The purpose of this study was to identify and compare aerobic microorganisms adheringto polished and tissue surfaces of temporary removable partial dentures at 24hrs and at one week ofdentures in use.

Materials and methods: A total of 31 patients visiting the department of Prosthodontics for temporaryremovable partial dentures without any known systemic and oral diseases were included in thestudy. Dentures were fabricated according to the standard prosthodontic procedures accepted in thedepartment. Each patient was examined twice, once after 24hrs of denture insertion, and thenat oneweekof use wherein plaque samples were collected from the polished surface and the tissue surface.The samples were taken to the microbiology lab for microbiological investigations within half an hourof sample collection and different microorganisms were identified.

Results: Microorganisms identified at 24 hours from polished and tissue surfaces of the dentureswere same in type and not significantly different in frequency of isolation. They were Streptococcusspp., Staphylococcus spp., Acinetobacteranitratus, Enterobacter cloacae, Pseudomonas aeruginosa,Citrobacterfreundii and Candida albicans.Those identified at one week of dentures in use, from the twosurfaces were same in type but three new types of microorganisms, Klebsiella pneumonia, Escherichiacoli, and Proteus vulgaris were also found. There was a significant difference in the isolation frequencyof Staphylococcus spp. from the two surfaces.

Conclusion: Same types of microorganisms adhere to both the surfaces but significant differences mayoccur in frequency of isolation. With time denturesfavour colonization of new microorganisms.

Key words: Adherence; denture microorganisms; denture plaque; surfaces of dentures; temporaryremovable partial denture

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